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4. Why did you need to wait for the column bed to be dry (the absence of buffer on the top of the column bed) before adding
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Ans 4) We need to wait for the column bed to be 'dry' before adding the protein so that it would not get mix with anything else to prevent it from diffusing in any other substance. There is requirement to add more buffer when protein mixture was loaded in the column to avoid it from drying. So that the molecules cannot pass through it.

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