Question

Please include:
RNA pol II, TBP, TFIIB, TFIID, TCF4. Mediator, BRE, TATA, Inr, DPE, E box

Question 5: (5 marks) Mediator is a protein complex that is able to act as a bridge between transcriptional activators and the TFIID-dependent RNA Pol II machinery. Draw what you would expect the promoter region to look like with Mediator acting at the promoter of a gene with an E-box 3000bp upstream of the transcriptional start site, which requires TCF4 for activation of transcription. Be sure to incorporate all information on TCF4 given to you and label all of the possible core DNA elements and proteins that could regulate transcription of this gene.

Answer 1

Answer:

The Schematic can be drawn as follows wst Activators TCF - 4 Mediator (ß-cate P300 TSS Promotie (transcriptional start site E-box (-3000 B-cat 130 TCFY ATG 1stCANNTG-3 그생 Promoter Topene transcribed

The Tef4 is a transcription tauta that recognises the 5' CANNTG 3' sequence in the E-box, which recruitus the other teanscription bartors like TCF4E (6) TCF5 to further the process of transcription. suppose the promoter is cdx1, so the genes under this promoter are transutbed Beta conteur and P300 (coactivati) by please rate my Ansuch

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