Question

Determine the sequence of a tetra peptide:

1)The second amino acid has a phenol in the side chain.

2)The amino acid at the amino terminal is acidic and the only acidic amino acid present.

3)Amino acid with CH3 side chain at C terminal.

2)Pepsin - cuts on the C-terminal side of phenylalanine, leucine, and glutamic acid. When digested with the pepsin the peptide gives a negative amino acid residue and neutral tripeptide

3)Cyanogen bromide- cuts on the C-terminal side of the methionine.

When digested by cyanogen bromide it gives a neutral amino acid residue, and negative tripeptide.

Answer 1

Let us tte that tetrapeptide as N-A-B- C N theNtemi nal amino aid, and teaminat amino adpectivel From given data Ciamino acid at the amino teaminal is acidic N, can be Aspartic acid· 07 Glutamic acid ii) second amino acid: aš phenal. 10 ha sde chain The only aminoac sid e chain S dhain phenol the yrosine the-Position of 'A

iv) Aminoacid th CH side choin i Alanine egan ogen bγ0m de cuts on the c- terminal Slde o2 he methismine a nsahve tri peptide an C his indicates that wilt be methionine (Yi). Pepsin will act on the c eminal o phengtalanine leucine and glutamic acíd onlg N tminal amino aid inou Letrapeptid e splutamic acid nob aspautic acid Hente the peptide chainille MetARa 2 CH 2 2 とH S- CIH NH 2