Question

1. Using the Activity 2 data from the Lab 6 module, fill in the table below, using "+" for growth or "-" for a lack of growth to indicate which strains were supported by which media. Deduce the amino acid that is required in the growth medium to support the growth of each strain. + Medium UMM+his UMM+lys VMM+tip Strain UMM Rich Amino acid requirement A B с D E F G

This is the data from activity 2

Answer 1

Strain	Medium
UMM	UMM+ His	UMM+Lys	UMM+Trp	Rich	Aminoacid requirement
A	-	+	-	-	+	Histidine
B	-	-	+	-	+	Lysine
C	-	-	-	+	+	Tryptophan
D	-	+	-	-	+	Histdine
E	-	-	-	+	+	Tryptophan
F	-	+	-	-	+	Histidine
G	+	+	+	+	+	No specific requirement

From the table we can infer that Strain A grows on Rich medium containing all aminoacids but when individual aminoacids are tested, it requires Histidine for its growth. Similarly we can get the data for rest of the strains.

Similarly strain G grows on any media without any requirement for specific aminoacid.

i have kept the order of strains as

A D

B E

C F G

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