Question

13. The figure in the below is the flow collected from healthy human. The total number of white blood cells is counted to be 5,000 cellis/uL. Provide your answers to following questions (34pts) cytometric analysis of red cell lysed blood sample Gating of A cells a:5 %| b:25% 50% c:30%| d:40% CD3 PE/Cy5 10% 40% . Geting of A cells | e:5%| f:50% g: 30 h: 15% CD3 PE/CyS (A) Calculate the total number of monocytes (cells/ul) (3 pt) (B) Calculate the total number of neutrophils (cells/uL) (3 pt). (C) Calculate the total number of lymphocytes (cellspL) (3 pt).

Answer 1

A) Monocyte count:

Total numbers of white blood cells are 5000 cells / µl

In this figure, monocytes are found to be 10% of total white blood cells.

So, 5000 X 10 /100 = 500 cells / µl

B) Neutrophil count:

Total numbers of white blood cells are 5000 cells / µl

In this figure, neutrophil are found to be 50% of total white blood cells.

So, 5000 X 50 /100 = 2500 cells / µl

C) lymphocyte count:

Total numbers of white blood cells are 5000 cells / µl

In this figure, lymphocytes are found to be 40% of total white blood cells.

So, 5000 X 40 /100 = 2000 cells / µl