Question

Short and clear answers please! Thank you

Question 2: Advances in DNA sequencing, 5 points A. 3 points. Imagine you are explaining the revolution in DNA sequencing to a relative. You're trying to explain to them why the price dropped one-million fold. The major key advance that lead to this drop in price is: B. 1 point. In Next Generation Sequencing, dsDNA linkers are added to each of the fragmented pieces of DNA. This linker enables the fragments to stick to the flow cell. What other function does the linker provide? (one sentence max): C. 1 point. Shotgun sequencing involves shearing the DNA into short fragments and sequencing them all at once. After sequencing the short fragments, the researcher must assemble the sequences (also called "reads") into a complete sequence. Does this approach require that the researcher start with many copies of a DNA template or is one enough? In providing your answer (yes or no), explain how the researcher assembles the reads into a complete sequence. Circle your answer: Many templates are needed One template is enough Provide a one sentence explanation for how the sequence reads are "assembled":

Answer 1

1. The advancements in the sequencing techniques like emergence of "next generation" high-throughput sequencing technologies has contributed most to the decline in the price of the sequencing to a greater extent. Apart from this the competition that stimulated the innovation has also led to the drop down of price.
2. This ds DNA linker provides the attachment of the fragments to the cell flow as a major function. But along with this it is also important as it binds the two DNA fragment and forms DNA fragment as whole nucleosome. This helps in the sequencing of long fragments.
3. In case of the shotgun approach for gene sequencing, it is recommended that one must use multiple copies of the DNA, so that even if anty of the fragment is not completely sequenced and shred in between, then there must be availability of another fragment with full sequence.

This will help the researcher to get the full contig without any gap. And there wil be whole gene sequencing possible.