What kind of solution should you use as a “blank” or “reference” solution if you wanted to quantify DNA that was in a solution of 25uM MgCl2? Provide an explanation for your choice.
Answer: The blank solution should be 25uM MgCl2 solution.
Reasons: In any analysis case, the blank solution should be that in which analyte sample is prepared. It is 25uM of MgCl2 here in the question.
What kind of solution should you use as a “blank” or “reference” solution if you wanted...
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If you have 72 mL of a 0.67 M solution and wanted to use it to make a 0.012 M solution, what would the total volume (in mL) of the new solution be?
Can you pleasee answer these questions regarding the experiment of DNA quantification. What is “blank” and why do we use it in DNA quantification? If you are told to analyze DNA samples that we extracted from buccal swabs, what would you use as a blank? 3. Explain in details: a. Concentration b. A260/A280 ratio c. A260/A230 Why are those measurements used, how do you know which concentration to expect if you used the commercial kit for DNA extraction, what is...
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1. Ifyou wanted to make a 4 M NaCl solution and you wanted a full Liter of it, how would you make it? Describe all of the steps. (Remember from lab, molecular weight of NaCl is 58.44) 2. Now describe how you would dilute this 4 MNaCl solution by a 1:10 dilution -how would you do this? Again, make 1 Liter. 3. Suppose you wanted a 2: 50 dilution of a stock solution, but you wanted 200 mL of it....
Suppose you have stock solution with a solute concentration of 35 mM. If you wanted to dilute this solution to make a total of 1 ml of a 7 mM solution what volume of stock _____ and what volume of water _____ would you use? Give your answer in micro liters (mu l), and show your calculations. Volume of stock _____ mu l Volume of water ______ mu l
(17) Suppose you have stock solution with a solute concentration of 10 mm. If you wanted to dilute this solution to make a total of 1 ml of a 3 mM solution, what volume of stock and what volume of water would you use? Give your answer in microliters (ul), and show your calculations. Volume of stock3 ul Volume of water · T ul
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JUST NEED PART C, A AND B ARE FOR REFERENCE A)Suppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and Na2HPO4. If the final solution was 0.17 M in KH2PO4, what concentration of Na2HPO4 would you need? (pKa for H3PO4, H2PO4−, and HPO2−4 are 2.14, 6.86, and 12.40, respectively.) (answer 0.234) B)Now assume you wish to make a buffer at the same pH, using the same substances, but want the total phosphate molarity ([HPO42−]+[H2PO4−]) to equal...