Question

(17) Suppose you have stock solution with a solute concentration of 10 mm. If you wanted to dilute this solution to make a total of 1 ml of a 3 mM solution, what volume of stock and what volume of water would you use? Give your answer in microliters (ul), and show your calculations. Volume of stock3 ul Volume of water · T ul

Answer 1

C1V1 = C2V2

C1 = 10 mM

V1 = ?

C2 = 3 mM

C2 = 10 mL

10 × V1 = 3 × 1

V1 = 0.3 mL = 300 uL

We need 300 uL of stock and (1000 - 300 = 700 uL) of water.

1 mL = 1000 uL

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