Question

(16) Suppose you have a stock solution with a solute concentration of 150 mm. If you wanted to dilute this solution to make a total of 50 ml of a 25 mM solution, what volume of stock __and what volume of water_ would you use? Give your answer in ml, and show your calculations. Volume of stock. 3 ml Volume of water . 7 ml

Answer 1

Stock Solution:

Concentration = 150 mM

Solution to be prepared:

Concentration of the solution to be prepared = 25 mM

Volume of the solution to be prepared = 50 mL

Calculations:

Stock                                              Prepared solution

M1V1                         =                         M2V2

150 mM X V1            =                25 mM X 50 mL

                V1                =              (25 mM X 50 mL) / 150mM

                V1                =             8.33 mL

Volume of stock = 8.33 mL

Volume of water = Total volume to be prepared – volume of stock

                                = 50 – 8.33 mL

                                = 41.67 mL

Volume of water = 41.67 mL