Question

if +Fit to page a. Setl is a histone methyltransferase that specifically methylates histone H3 at the lysyl residue located at the fourth position (H3K4) within the exposed tail. Set1 can mono-, di-, or trimethylate H3K4 and this degree of methylation can impact nucleosome positioning and thus gene expression levels a. SETI is the gene that encodes Set1. It is being cloned into the pRS406 vector shown below: The multiple cloning site (MCS) is contained specifically in which region of this plasmid: could be grown on The SETI gene contains (hint: find number of amino acids) a. This s particularly usefu for select process where cells media source. (3 pts) b. (1pt) c. The insert and pRS406 plasmid were digested with the restriction enzymes Sacil and Xhol to create the homologous sticky overhangs necessary for cloning. Using the pRS406 vector map, determine the size of the linearized pRS406 plasmid produced from this double digest. (4 pts)

I need with questions 4d and 4e.

Answer 1

4d -

SET1 proteins have 1080 amino acids. So the number of nucleotides that are present in the gene will be 1080*3= 3240.

Length of the pRS406 plasmid is 4384 base pair. So total length of plasmid + insert  = 3240+4384 = 7624 base pair.

It will little less than this as some bases will be removed during digestion of the plasmid with the restriction endonuclease.

4e - Bacterial cells have all the genes that are required for their growth. the pRS406 plasmid has an ampicillin resistance gene. If we grow the transformed cell in the presence of ampicillin, the cell will utilize this plasmid.

It has lac promoter which can be induced by IPTG and plasmid will start expressing the protein. But it may produce a functional protein or not is depend on the post-translational modification that is required for the activated protein.

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