Question

Please Help!!!

The researchers set up the following experiment: 1. Each yeast strain is diluted in phosphate buffer solution so that there is the same concentration 2. Lysis buffer, which dissolves the cell membrane and releases the cytosol into the solution, is 3. ONPG is added to each tube. After 15 minutes, the researchers use a spectrophotometer to 4. The Ago is used to calculate the Activity Units of each tube, measured in velocity/mL. Activity of cells in each tube. added to each tube. measure the A42o for each reaction. units are a measure of how much active enzyme there is present in a solution. The higher the Activity Units, the more active enzyme is present. Their data is shown in the table below. Yeast Strain Activity Units (velocity/mL) 3.6 95.3 241.7 6.2

Answer 1

5. Independent viariable - Yeast strain

6. Dependent variable - Enzyme activity

7. Positive control - A strain

8. Negative control - E strain

9. Standardized variables - Phosphate buffer, Lysis buffer and ONPG

10.

Enzyme Activity in Different Yeast Strains a200 o 100 0 50 2 Yeast Strain

11.

a) Repressor: It is a Protein

b) Operator: DNA segment

c) Activator: It is a Protein

d) Enhancer: DNA segment

It is a Pleio that binds to the openalor vegion ooDler i Ve ences lhe bso l esny Yeprees.rg it e qene exion CYeases The ene5 Can be a parliculas ene oilorc