Question

D Question 8 The ter inhuman genome via the rigen cloud has enclosed the New York Metropolitan area, activating the transcription factor IG. A BIO3320 friend of yours is not inhuman, but really wants some superpowe rs. For an independent study, they isolated a small amount of IG and determined on every behavioral tests they could think of. To produce IG for personal use, friend obtained a complete genomic clone of the IG gene, connected it to a strong that it can make mice perform better your ter and introduced the plasmid into E. Coli cell You find your friend crying in uced in the cells. What is the likely the lab, devastated to find that no IG was prod explanation for this failure? Sigma promoter cannot bind to a eukaryotic TATA box Your friend should have started with an IG cDNA instead of a genomic clone. Your friend is an over e Eukaryotic proteins cannot mutant for the dumbass protein be produced by an E.Coli cell Eukaryotic IG transcript is targeted for destruction by E. Coli miRNAs MacBook Air

Answer 1

Answer:

What is the likely explanation for this failure?

• Sigma promoter cannot bind to a eukaryotic TATA box (incorrect, sigma factor which is a bacterial transcription factor is required for transcription and it enables binding of RNA polymerase to gene promoter).
• Your friend should have started with an IG cDNA instead of a genomic clone - Correct Option (Eukaryotic mRNA undergo post translational modification like capping, polyadenylation, and splicing. Eukaryotes also have a slightly different codon usage. Therefore, cDNA libraries are used to express eukaryotic genes in prokaryotes.)
• Your friend is an over expression mutant for the dumbass protein
• Eukaryotic proteins cannot be produced by an E.coli cell
• Eukaryotic IG transcript is targeted for destruction by E.coli miRNAs