RNA processing is determined by phosphorylation patterns in the C-terminal repeat domain (CTD) of the RNA polymerase II. Explain in a mechanistic manner how a deficiency in the enzyme CTDK-1 affects RNA processing.
There are many kinases in saccharomyces cerevisiae that phosphorylate the CTD of Rna polymerase II. CTD is essential and it has vital functions in initiation and elongation of transcription as well as in processing of MRna. The function of the CTD in these different processes appeares connected to phosphorylation of CTD, which changes through transcriptionery cycle. In saccharomyces cerevisiae, four enzymes are thre for CTD kinase activity. That os, Kin28- Ccl1, Srb10- Srb 11, CTDK-1, and Sgv1/Bur1 /Bur2. CTDK-1 is responsible for proper processing of a subset of yeast transcripts.
RNA processing is determined by phosphorylation patterns in the C-terminal repeat domain (CTD) of the RNA...
B. The C-terminal domain (CTD) of RNA polymerase II has a repeating structure. Phosphates can be attached to serine residues within the repeats. What are the various roles that CTD phosphorylation can play? How might the repeating nature of the CTD be important for those roles? How can you test your ideas experimentally? Cite your work
1PT A. Describe the structure and function of the carboxyl terminal domain (CTD) of RNA polymerase II. 1PT B. Give a functional definition of a gene.
Describe how the carboxy terminal domain (CTD) of RNA polymerase II is involved in initiation and elongation of transcription. HTML Editori B I VA - A - Ix E 1 1 1 x X, DE T TH 12pt Paragraph Tube O words
Which of the following mRNA processing factors associates with the CTD of RNA polymerase II? A. Splicing factors B. Polyadenylation factors C. Capping proteins D. siRNAs
What keeps TFIIF from stimulating a specific phosphatase to remove the phosphate groups from the CTD while RNA pol II is still in the preinitiation complex (PIC)? Contact with mediator through SRB4 Contact with TFIIB Contact with TFIIA through the large subunit (TFIIA alpha) Cdk8 of mediator represses TFIIF activity Which of the following would most likely NOT be found in the promoter of a typical housekeeping (constitutive) gene? TATAA Initiator region Enhancer element CpG island How is the mediator...
can someone explain how to answer this with reasons why
30-45 mins after estrogen addition: Acchyiase (HAT, odde auhye 60-90 mins after estrogen addition: Reauitment ef Poy to open 7. T identify the Gene X DNA element responsible for regulation by a Growth Fagor, you perform a linker scanning experiment in which you mutate 10 bp Tegions centered around positions -205 to-5 in the Gene X promoter/promoter- proximal region (wt wild-type promoter). The promoter variants were tested for their ability...
1. Bacteria such as E.coll and staph belong to which Domain? A. Animalia B. Eukarya C. Archaea D. Bacteria 2. In general terms, prokaryotic cells are cells that? A. Have a nucleus B. Do not have a nucleus. 3. Think about the name E. coli 4X56. In that name which refers to the serotype? A E B. coli C4X56 4. Which of the following best describes the type of bacteria that are important to ecology, and once were thought to...