When cells are centrifuged, after ethanol has been added, DNA will be found within the ________....
When cells are centrifuged, after ethanol has been added, DNA will be found within the ________.
|
supernatant. |
||
|
pellet. |
||
|
chelating agent |
||
|
detergent. |
Homework Answers
Pellet is the correct option.
Explanation : Generally in the process of DNA isolation the ethanol is added so that it is able to precipitate the DNA in form of pellet by removing all the contaminated salts used during isolation process. Wheras supernatant contains all the remaining buffer impurities along with alcohol .The alcohol is used is chilled alcohol so that DNA is prevented from further denaturation. Later after the pellet formation pellet is dissolved in nuclease free water and stored in cold temperature for further use or checked in agarose gel electrophoresis and by nanodrop method for obtaining pure DNA without any contamination.
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