Question

The Edmann degradation method is used to break proteins into smaller peptides. True False

Answer 1

Edmann degradation method is used for protein sequencing.

itbsi used to label the amino terminal residue of the protein chain and to cleave that residue from the main protein chain without disturbing the remaining peptide bond .

First step-Coupling

phenylisocyanate react with the alpha aminoacid of N terminal of protein chain.

2nd step:cleavage

String acid cleave the first peptide bond and release the residue from the main chain.

3rd step:conversion

Residue is processed from peptide by using organic solvent and converted into more stable configuration

4th step-Analysis

Analysis of the residue with the help of chromatograohy