Question

21. What does restrictive temperature mean? What does nonrestrictive temperature mean?

22. What function does the protein dynamin specifically perform in the cell?

23. What is happening to the dynamin protein in the shibire mutants at the molecular level at the restrictive and nonrestrictive temperatures? Think in terms of protein structure, function, and activity.

24. Find and describe one other human conditional allele (not PKU).

25. One of the overarching themes of this lab and accompanying class is the Central Dogma of Molecular Biology. How does this lab fit into the Central Dogma?

Answer 1

I will be answering the first two questions.
21.
Restrictive temperature:
Is that temperature at which the temperature sensitive mutation expresses a mutant phenotype

Permissive or non restrictive temperature-
Is the temperature at which a temperature sensitive mutant allele is expressed to be the same as the wild-type allele.

2.
Dynamin is a GTP-ase protein responsible for the transport of synaptic vesicle(Endocytosis)- synaptic vesicle transport. The particular family of dynamin have other functions like
* Microbial pathogenic resistance
* cytokinesis ( division of cells)
* Organelle division

Thank you...