Question

nuclear transplantation site-specific recombination nuclear microinjection transposon cloning homologous repair ecdysone locus control region insulator sequences targeting germline chimeric/mosaic 1. In 1974, Rudolf Jaenisch created the first genetically modified animal, a mouse, by introducing novel genetic information into the Mus musculus Transgenic animals are often generated by , where DNA transgenes are inserted into the nucleus of a fertilized egg cells. These engineered eggs will give rise to progeny that possess one copy of the transgene and must be bred to yield animals that are homozygous for the transgene. 3. When only certain cells in an organism contain an integrated transgene and other cells do not, the transgenic organism is said to be 4. The location of an inserted transgene can drastically affect its subsequent expression. To compensate for location effects, appropriate regulatory elements such as _(also called boundary elements) can be engineered into the transgene to optimize its expression. If a gene is flanked by these sequences, it is protected from the effects of any distant regulatory elements. 5. Enhancer sequences control nearby genes in a dominant manner and can also be engineered into transgenes to combat location effects. One example is the in front of the B-globin gene cluster, which confers high-level expression for several genes and is independent of chromosome location. _ vectors rely on homologous recombination to insert a transgene into a host chromosome at a specific location. The CRE/LOX system is an example. 7. The tissue specific or conditional expression of a transgene can be accomplished by using a hormone responsive promotor such as that induced by , an insect hormone. 8. that can be used to introduce genetic The P element in drosophila is a changes into the important fruit fly animal model. 9. A group of genetically identical animals may be cloned by - -, a process in which the nucleus from a somatic cells of the same donor are transplanted into an enucleated egg cells (egg cell without nuclei). involves using genetic technology to make an exact genetic duplicate of 10. The process of an animal.

Answer 1

Germline Nuclear microinjection Chimeric I Mosaic Insulator locus control region 6. site specific recombination 70 Ecdysone Transposon Wani Nuclear transplanation 10. Cloning.