Question

H26.35-Level 2 The compound succinimidyl trans-4-(maleimidylmethyDcyclohexane-1-carboxylate shown below is a common commercial linker that biotechnologists use to connect a protein with an amino reactions are utilized in this method of cross-linking? group with another protein with a -SH group. What type of chemical O-N Maleimidyl fragment Succinimidyl fragment Succinimidyl fragment reacts with-SH groups by nucleophilic acyl substitution, maleimidyl fragment reacts with a mino groups by Michael addition Succinimidyl fragment reacts with amino groups by nucleophilic acyl substitution, maleimidyl fragment reacts wit h -SH groups by Michael addition Succinimidyl fragment reacts with-SH groups by Michael addition, maleimidyl fragment reacts with amino groups by nucleophilic acid substitution Succinimldyl fragment reacts with amino groups by Michael addition, maleimidyl fragment reacts with-SH groups by nucleophilic acyl substitution

Answer 1

H26.35 The correct answer is B.

Succinimidyl trans-4-(maleimidylmethyl)cyclohexane-1-carboxylate (SMCC) is a heterobifunctional, crossslinker which facilitates the covalently link two target groups. The succinimidyl ester chemically reacts with primary amines and the maleimide can react with available thiols.