Question

20) Mouse 3T3 cells will continue dividing in culture A) until all nutrients are consumed from the media B) as long as a cells maintain contact with each other C) until cells stop dividing due to contact inhibition D until 7 monolayers are formed B) A & B

homework problems, answering all or one would be a huge help

Answer 1

20)c) until the cell stops dividing due to contact inhibition.Swiss 3t3 cells can be inhibited by temazepam and other benzodiazepines.

11.A) 5'GGATATCC3' this is restriction enzyme cut in 8 nucleotide sequence.

A two-dimensional 500-MHz 1H-NMR study of two oligonucleotides, d(GGATATCC) and d(GGM6ATATCC), is presented in which we have investigated the effects of adenine methylation. The two-dimensional nuclear Overhauser spectra (NOESY) show that both oligonucleotides adopt a normal right-handed B-type helix and one-dimensional nuclear Overhauser enhancement (NOE) studies demonstrate th at any difference in conformation must be small. However methylation drastically slows down the helix in equilibrium coil exchange which becomes slow on a proton NMR time scale. While d(GGATATCC) fits a two-site exchange model, d(GGM6ATATCC) does not and we invoke the presence of a third species which may be an intermediate in helix formation. NMR and ultraviolet spectroscopy show that methylation destabilizes the helix, measured by the melting temperature and enthalpy of dissociation.