Question

Demonstrate here your ability to compare PROTEIN sequences by clustal Omega. Print out the analysis and phylogeny for PREPROINSULIN from the following vertebrate species

Rattus norvegicus

Mus musculus

Macaca fascicularis

Bos taurus

Cavia porcellus

Gallus gallus

Oncorhynchus keta

Homo sapiens

Danio rerio

Rana pipiens

Sus scrofa

Pan troglodytes

Felis catus

Xenopus leavis

mutation occurs at a very slow rate and is theoretically random across a genome. Yet the sequence variation in this gene is NOT random. Explain briefly incorporating your understanding of the physiological processing and functions of preproinsulin=>proinsulin=>insulin. You might want to use highlighters to highlight the various domains of the molecule. Interpret very briefly and concisely the phylogenetic tree you will also print out.

Answer 1

multialignment done by clustal omega after retrieving protein sequence from uniprot. screen shot of alignment and phylognetic tree is given below.

preproinsulin is secrated by beta cell of pancreas in inactive form. it gets signal peptide cleaved by proteases that premature into proinsulin further cleavage and disulfide formation transform into mature active insulin form.

Results for job clustalo-120160727-204427-0685-58020103-oy Ali lignments s Result Summary Phylogenetic Tree Submission Details Download Alignment File Hide Colors Send to ClustalW2_Phylogeny CLUSTAL 0 (1.2.2) multiple sequence alignment trlQ2QAJ9 1Q2QAU9_DANRE sp l P04667 I INS_ONCKE trlQ9I8071g9I8Q7_LITPI sp I P01329 INS_CAVPO trIQ6XVM1 I 26XVM1_CHICH sp| P01322 INS1 RAT sp l P01325| INS1_MOUSE tr IQ52PU3 1 Q52PU3_FELCA sp l P013171 INS_BOVIN sp l P01315 I INS_PIG sp I P30406I INS_MACFA sp l P01308 1 INS HUMAN MVLLLQASVLILLLASLP--GSQSSPSQHLCGSSLVDALYLVCGPRGFFYTNRGRRDLET MAFWLQAASLLVLLALSPG--VDAAAAQHLCGSHLVDALYLVCGEKGFFYTPKRDVD MALWIQCLHIAVLLSLFTPT-LKTEDNOYLCGSHLVEALYMVCGDRGFFYSPRSRRDLEQ MALWMHLLTVLALLALWGPNIGQAFVSRHLCGSNLVETLYSVCODDGFFYIPKDRRELED -LPLLALLVFSGPGTSYAAANQHLCGSHLVEALYLVCGERGFFYSPKARRDVEQ MALWMRFLPLLALLVLWEPKPAAFVKQHLCGPHLVEALYLVCGERGFFYTPKSRREVED MALLVHFLPLLALLALWEPKPAFVKHLCGPHLVEALYLVCGERGFFYTPKSRREVED FVNQHLCGSHLVEALYLVCGERGFFYTPKARREAED MALWTRLRPLLALLALWPPPPARAFVNQHLCGSHLVEALYLVCGERGFFYTPKARREVEG MALWTRLLPLLALLALWAPAPAQAFVNQHLCGSHLVEALYLVCGERGFFYTPKARREAEN MALWMRLLPLLALLALWGPDPAPAVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAED MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAED trlQ2QAJ9 1Q2QAU9_DANRE sp l P04667 I INS_ONCKE trlQ9I8071g9I8Q7_LITPI sp I P01329 INS_CAVPO trIQ6XVM1 I 26XVM1_CHICH sp l P013221 INS1_RAT spl P0 13 251 INSI MOUSE tr IQ52PU3 IQ52PU3_FELCA sp l P013171 INS_BOVIN sp | P01315 | INS_PIG sp l P30406I INS_MACFA sp P01308 I INS_HUMAN LLALLSN-LAGYEAADADPLKEKVMKMKRGIVEQCCHRPCTIYHLEDYCS PLIGFLSPKSAKENEEYPFKDQTEMMVERGIVEQCCHKPCNIFDLONYCN PLVNGLQGSELDEMQVQSOAFQKRKPGIVEQCCHNTCSLYDLENYCN PQVEQTELGMGLGAGGLOPLALEMALQKRGIVDQCCTGICTRHOLQSYCN PL QKRGIVDQCCTSICSLYQLENYCN PQVEQLELGGSP--GDLQTLALEVARQKRGIVDQCCISICSLYQLENYCN LQGKDAELGEAPGAGGLQPSALEAPLQKRGIVEQCCASVCSLYQLEHYCN PQVGALELAGGPGAGGL-EGPPQKRGIVEQCCASVCSLYQLENYCN POAGAVELGGGL--GGLOALALEGPPQKRGIVEQCCTSICSLYQLENYCN PQVGQVELGGGPGAGSLOPLALEGSLQKRGIVEQCCISICSLYQLENYCN LQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCISICSLYQLENYCN PLEASE NOTE: Showing colors on large alignments is slow

Results for job clustalo-120160727-204427-0685-58020103-oy Alignments Result Summary Phylogenetic Tree Submission Details Phylogenetic Tree This is a Neighbour-joining tree without distance corrections. Download Phylogenetic Tree Data Branch length:Cladogram Real trlQ2QAJ9IQ2QAJ9_DANRE 0.27094 spl P04667|INS, ONCKE 0.24362 trlQ9I8Q71Q918Q7_LITPI 0.23944 trl Q6XVM1| Q6XVM1_CHICK 0.08233 trl Q52PU3 Q52PU3_FELCA 0.08537 sp P01317 INS_BOVIN 0.06272 splP013 1511NS-PIG О.05378 ー sp P01329 INS_CAVPO 0.22976 spl P01322 INS1_RAT 0.02414 sp P01325 |INS1_MOUSE 0.04067 spl P30406|INS_MACFA 0.00602 sp I P01308|INS_HUMAN 0.01217 Tree Data