Question
Assume a starch agar plate was inoculated and incubated as indicated in the exercise,
and that you did the same for your unknown.
• Assume that, after incubation, Gram iodine solution was added to completely cover the
plate as indicated in the lab exercise.
• Record in Table 4 the appearance of the agar immediately around each bacterial streak
(not the bacterial growth itself) shown in Fig. 4:
o D = agar surrounding growth is Dark; L = agar surrounding growth is Lighter
• In the last column of Table 4, conclude if the organism hydrolyzed starch.

Figure 4. Escherichia coli Bacillus Subtilis TABLE 4: Color of Agar Around Growth S tarch Hydrolysis (Y or N) Bacterial Speci
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Answer #1
Bacteria color around growth starch hydrolysis
Bacillus subtili Clear zone Y
Escherichia coli No zone or no clearing N
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