The Lac operon is a classic example of regulatory logic. Summarize the details of the control of the Lac operon pathway, and also explain how this is advantageous for the organism as a whole.
Regulation of lac operon is governed by a repressor which binds to its operator region which prevents the expression of the downstream genes. This repressor binds only when lactose is absent. When sufficient lactose is available it enters the cell converts allolactose which binds to the repressor which releases from the operator inducing transcription.
Another set of regulation is governed by a protein called CAP (cAMP activated protein) which is available when glucose is absent. CAP binds to the promoter region facilitating transcription.
The Lac operon is a classic example of regulatory logic. Summarize the details of the control...
Describe/draw the lac operon (including both the regulatory regions and the coding regions) and explain how its expression is regulated. Is it inducible or repressible? Explain why you answered this way.
ACTIVITY #2: Operon control of gene expressionComplete the comparison of the lac Operon and trp Operons as a means of gene regulation. lac operorn trp operon Regulates production of Name of Regulatory gene" and what it produces What binds to the operator & when does this occur: Structural genes controlled by the operon and how their expression is controlled: High levels of what substance affects the operon how?
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
61. The lac operon a. is an example of negative control. b. is affected by catabolite repression c. produces lactose. d. is an example of a regulon. e. a and b Bonus questions: nepxample of its use. (0.5 pts) Describe biotechnology and list only one example of its use. es that (0.5 pts) Why are bacteria so diverse and list one example of such diversity?
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In the lac operon, positive control occurs through the presence of the CAMP-CAP complex. This complex is not available in the presence of glucose. this would mean that: When lactose is present, the lac operon will be activated only if glucose is absent When lactose is absent, the lac operon will be activated only if glucose is also present When lactose is present, the lac operon will not be activated regardless of glucose presence When lactose is present,...
Can someone please explain the following lac operon mutation regulatory effect on transcription: 1) lacOCZ+Y+/lacO+Z+Y+ = Lacc 2) lacOCZ-Y-/lacO+Z+Y+ = Lac+ 3) lacI-D/lacI+ = Lacc 4) lacIS/lacI+ = Lac-
1. Describe in detail how both positive and negative regulation control expression of the lac operon in bacteria. In doing this, also convey understanding of how and why expression of this operon is controlled two ways (positive and negative). Also answer, what are the energetic benefits to the cell of operon regulation? 2. What is horizontal gene transfer? Describe in detail the three mechanisms of horizontal gene transfer. What are the evolutionary advantages and costs of horizontal gene transfer. I...
1. (1 pt) If a gene is repressible and under positive control, A. Is the regulatory protein an activator or repressor? B. Explain how an effector molecule, which binds to the regulatory protein, alters the regulatory protein’s ability to regulate expression of the gene. 2. (1 pt) Cis and trans are two terms used to describe mutations. A. Explain the molecular difference between these terms. B. indicate which type of mutation (cis or trans) is dominant to wild-type and which...
For this assignment you will complete an illustration(s) (by hand) of how the lac operon works. You should be sure to include details in your drawing such as the names of relevant structural genes (lac Z and lac Y) and controlling sequences (operator, promoter, repressor). 1. Show the influence of lactose (in the form of allolactose) on the repressor protein. 2. Show what effect the primary structural genes have on lactose. 3. Show how glucose levels can influence the expression...
4. Explain how the genes that control galactose metabolism in yeast are regulated in yeast. How does it differ from the regulation of the lac operon in bacteria? please be detailed in your answer