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Describe the production of influenza vaccine using chicken eggs.  What are the limitations to this production method?

Describe the production of influenza vaccine using chicken eggs.  What are the limitations to this production method?

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By using chicken egg two type of influenza vaccine ara made that is inactivated killed vaccine or live attenuated vaccine. Virus are injected into the fertile chicken egg and kept for incubation for 2 to 4 days to allows viral replication in cell. After incubation virus containing fluid isolated from egg. Then for killed vaccine virus are killed and inactivated and virus antigen is purified in this way killed inactivated vaccine prepared. In case of live attenuated vaccine virus particle are attenuated by diffrent chemical method that is their disease causing ability destroyed.

Limitations are; Only fertile egg required for production, sometime get contaminated due to improper technique. Isolation is also difficult task. Sometime virus not replicated. Chances to infect person is high. These are the limitation of influenza vaccine by chicken egg.

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