how many cells/ml are in the original sample?
can you please explain
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how many cells/ml are in the original sample?
can you please explain
5 ml 10 ml...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four grams of soil are added to 36 ml sterile water and mixed well. 0.1 ml of this mix was added to 9.9 ml sterile water. This was diluted by 4 successive 1/10 dilutions. 1.0 ml from the last dilution was used to prepare a pour plate. After incubation 289 colonies were counted. Calculate the # CFU/g soil. 2) A 0.1 ml aliquot of a...
1)How many microliters of original sample are required to produce a final dilution of 10-2 in...
1)How many microliters of original sample are required to produce a final dilution of 10-2 in a total volume of 5 mL? 1 microliter is 10-6 L or 10-3 mL. 2)A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 197 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture?...
6) How many bacteria would be in 1 ml of the original sample if the 108...
6) How many bacteria would be in 1 ml of the original sample if the 108 dilution has 200 colonies? 7) What criteria must your plates meet in order to be considered "countable" ? How many bacteria per milliliter of sample would be "acceptable" to the FDA? - 86 -
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
Please show how to do these enumeration questions by showing the work. What is the dilution...
Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the Plate Count Method, what final dilution factor would be needed to produce countable plates? Show your work. (B) Describe a dilution scheme (how many tubes, what volume in each tube, what DF is achieved in each step) that uses only the 9-mL blank diluent tubes to achieve the dilution needed for this FDF.
can u please help me with question 10. 10. Counting Cells a. You have 18 cells...
can
u please help me with question 10.
10. Counting Cells a. You have 18 cells in 100 Nano litters (18 cells in 0.1 microliters). What is the total of cells in 1 milliliter (ml)? b. You have a sample of RBC (Red Blood Cells). Since there are very tiny we are using only the center square in the figure. After counting under the microscope you have you find 82 cells inside the black area only. How many RBCs do...
can u please help me with this 10. Counting Cells a. You have 18 cells in...
can
u please help me with this
10. Counting Cells a. You have 18 cells in 100 Nano litters (18 cells in 0.1 microliters). What is the total of cells in 1 milliliter (ml)? b. You have a sample of RBC (Red Blood Cells). Since there are very tiny we are using only the center square in the figure. After counting under the microscope you have you find 82 cells inside the black area only. How many RBCs do you...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates,...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
6) How many bacteria would be in 1 ml of the original sample if the 108 dilution has 200 colonies? 7) What criteria must your plates meet in order to be considered "countable" ? How many bacteria per milliliter of sample would be "acceptable" to the FDA? - 86 -
can
u please help me with question 10.
10. Counting Cells a. You have 18 cells in 100 Nano litters (18 cells in 0.1 microliters). What is the total of cells in 1 milliliter (ml)? b. You have a sample of RBC (Red Blood Cells). Since there are very tiny we are using only the center square in the figure. After counting under the microscope you have you find 82 cells inside the black area only. How many RBCs do...
can
u please help me with this
10. Counting Cells a. You have 18 cells in 100 Nano litters (18 cells in 0.1 microliters). What is the total of cells in 1 milliliter (ml)? b. You have a sample of RBC (Red Blood Cells). Since there are very tiny we are using only the center square in the figure. After counting under the microscope you have you find 82 cells inside the black area only. How many RBCs do you...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
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