Question

9. The following measures may improve chromatography efficiency by mitigating peak broadening EXCEPT for (4 pt) a. decreasing the particle diameter in a column b. increasing the temperature (only in GC) C. optimizing the mobile phase flow rate d. reducing the thickness of the immobilized stationary layer e. reducing the length of a tube to introduce the sample

Answer 1

All the following measure may improve chromatography efficiency by mitigating peak broading except option (a). decreasing the particle diameter. We cannot change the particles diameter in column it is fixed for a definite molecule.