You are interested in examining T cell activation during an immune response in vivo. You are...
You are interested in examining T cell activation during an immune response in vivo. You are given: anti-Bcell, anti-T cell, anti-CD4, anti-CD8, and anti-IFNg antibodies.
Draw a simple gating strategy (plots) to show how you would measure T cell activation in a spleen. only draw ONE gate per plot draw cells as dots or large circles (or peaks for one parameter plots) draw an arrow to the final gate where you would expect to see protein expression differences for activated vs unactivated T cells
Homework Answers
a) lymphocytes will be selected from SSC-FSC plot, b) gate on only T-cells c) bivariate plot between IFN-gamma and CD-4 or CD-8. stimulated cells will be positive for both CD-4/CD-8 and IFN-gamma.
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You are interested in examining T cell activation during an
immune response in vivo. You are...
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A) lymph follicles lymph nodes C) lacteals D) lymphatics 4) An advantage to adaptive immunity is (A) its memory cells that provide quicker, larger and more efficient immune response upon second and subsequent exposure to an antigen B) its need for several cells to be activated over several days on first exposure C) the ability of its individual cells to respond to many different pathogens D) the use of antibodies that cause cell lysis and kill invading cells 5) A...
Case 3a: Immune Cell Mutiny You are a doctor working in clinic in Kenya. You are treating a pregnant, female patient who has had the following recurrent symptoms for the past 9 months: fever, fatigue, sore throat, swollen glands, night sweats, diarrhea, and weight loss. When you interview the patient she tells you that her husband recently tested positive for HIV. However, he told her not to worry because HIV cannot be transmitted during heterosexual intercourse. Alarmed that your patient...
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