Question

6) Many scientists doubted that shotgun sequencing would work, even with the smallest genomes, because: a. There would not be overlaps between the different mini-sequences. b. Computers would be unable to handle the huge amount of data generated by a shotgun sequencing project. c. Small prokaryotic genomes contain large amounts of repetitive DNA. d. No method existed for breaking genomic DNA into random fragments. 7) In a PCR-RFLP assay, enzyme digestion revealed three bands (152+187+462bp) in AA animals, four bands (83+104+152+462bp) in CC animals and five bands (83+104+152+187+462) in CA animals. How many polymorphic cut sites exist within the amplified product? a. 1. b. 2. c. 3. d. 4. 8) PCR-RFLP technique involves: a. PCR amplification. b. Restriction digestion. c. Gel electrophoresis. d. All of the above. 9) A Bi-PASA PCR reaction requires: a. One oligo. b. Two oligos. c. Three oligos. d. Four oligos. 10) AFLP technique requires: a. Restriction enzymes. b. T4 ligase. c. Taq polymerase. d. All of the above.

Answer 1

6. A

7. 3

8. All of the above

9. B. Two oligo

10. All of the above
1. Restriction of genomic DNA and ligation of adaptors to restriction fragments.
2. preselective PCR amplification by Taq polymerase.
3. selective PCR amplification, reducing further fragment number.