Question

You are investigating an autosomal recessive cencition in an extended family. The gene responsible for the concition is known, but the specific allele in the family under study is uncharacterized Part A As part of your research, you examine mRNA expression for the parents, their two unafTected children, and their affected chid with a Northern blot using a ful-length cDNA probe Your results, shown here, are as folows: . Both parents have low, but detectable, mRNA expression. The unaffected children have either nomal or low mRNA expression The affocted child has no detectable mRNA expression. What possible molecular defect could explain these results? Select every legitimate possibility from the choices below. The recessive allele could be a mutation in regulatory ONA that prevents chromatin from being remodeled from a losed to an open stae The recessive allele could be a mutation in a splice donor or acceptor sequence that prevents proper mRNA splicing, leaving an intron in the spliced transcript The recessive allele could be a mutation in the promoter region that prevents RNA polymerase II from binding The recessive allele could te a premature stop codon that prevents the translation of a full-Hength protein The recessive allale could te a mutation in the transcribed region that makes the mRNA unstable and causes it to degrade. Correct Your observation that the affected child has no detectable mRNA has several poeeible explanations. You decide that further experiments are needed to distinguish between them.

please Answer part B. Thanks.

Answer 1

Reset Help Promoter does not bind RNA pol Regulatory DNA will not remodel mRNA degrades Regulatory DNA Promoter Transcribed region Yos Affected child Affected child Affected child 2 Older brother Older brother Older brother Older brother Affected child Older brother Older brother Affected child Older brother Older brother Affected child Older brother