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In the fourth mutation you simulated a point mutation by changing the 10th nucleotide from T...

In the fourth mutation you simulated a point mutation by changing the 10th nucleotide from T to A. What type of mutation was this?

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Changing the 10th nucleotide from T to A is base substitution mutation which is a form of point mutation. A substitution mutation is the mutation that exchanges one base with another such as replacement of T by A or C by G etc. Such a substitution could change a codon to one that encodes a different amino acid and cause a small change in the protein produced.

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