How would you make 1 mL of a 1 to 25 dilution of yeast crude extract? Be specific.

How would you make 1 mL of a 1 to 25 dilution of yeast crude extract?...
1.How would you make 1 mL of a 1 to 25 dilution of yeast crude extract? 2. Enzyme activity measures: A)The number of polypeptide molecules B)The number of substrate molecule C)The energy used in the reaction D)The rate at which substrate is processed by the enzyme
How would you make 120 mL of a 1 to 25 dilution of a yeast extract? I'm really not understanding this process so steps/explanations would be nice. Thanks.
o Section 5 You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given distilled water, test tubes that hold a maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks on the figure to answer this question. mi 5 ml ml ml Iml a ml mi HO Yeast suspension Dilution: Dilution: Dilution: Dilution:
A crude cell extract containing 2 g.L -1 of protein was assayed for enzyme activity using an excess of substrate. It was found that 5 ml of the solution converted 25 µmoles of substrate to product over a period of 2 minutes. (i) Calculate the specific activity of the enzyme. The crude extract was subjected to anion exchange chromatography followed by gel filtration. After anion exchange the specific activity had risen to 226 µmoles.min-1 .mg-1 , and after gel filtration...
Explain how you would make a 1/1000 dilution of a bacterial suspension if you only had 3 ml aliquots of BHI broth (diluent) available. What size pipetman will you need to use to make this dilution?
I want to separate a protein from a crude yeast extract by ion exchange at pH 7.4. Should I use a DEAE column or CM cellulose column? The isoelectric point (pI) of the protein is greater than the pH (pI = 9). Explain.
You have 25 ml of a yeast culture that contains 8 x 10^9 cells/ml. How would you dilute this culture so that you have exactly 5 ml of culture that contains 2 x 10^2 cells/ml?
Show how you would make up a 10-5 dilution, given 1 ml pipets and 99ml water blanks. Your original samples is undiluted milk.
Question 6
Lab 16 Report 5. You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given tap water, test tubes that hold maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks (Red and Blue) on the figure to answer this question. 1ml 1ml 1ml 1ml 1:10 1:100 1:1000 1:10000 my mi water...
You need to make a 1:5 dilution of your mitochondria extract in PBS buffer. You want a total volume of your diluted sample to be 50 microliters. a. what volume of mitochondria extract do you need? b. what volume of PBS buffer do you need?