Question

biochem question

9. Just as for humans Pb2+ is toxic to bacteria. Many species have a P-type ATPase that exports Pb2+ from the cytoplasm to the exterior of the cell. Using the diagram below, show how this transporter works. The cell membrane is going up and down; the cytoplasm is to the left and the exterior is to the right. The ATPase is the box with a V-groove. The groove faces the cytoplasm in the top left case. The cytoplasm side of the protein and its channel are indicated the heavy lines. At step 1 two substrates bind, indicated by the two blanks. After filling in the blanks, show where these substrates reside on the enzyme. Use a circled P to indicate phosphate groups. Continue for the other steps. For steps 3 and 6 add a word to describe the event. cyt out . IF V RY K R

this is all the information that was given

Answer 1

1. Pb²⁺ and ATP (Binding)

2. ADP (Hydrolysis)

3. Eversion: Act of turning inside out

4. Pb²⁺ out

5. Phosphate (Pi) and H₂O (Hydrolysis)

6. Eversion

Hydrolysis Cytoplasm T P6t2 ATP 7 ADP ADP Eversion ③ Eversion Hydidysis IL HOY