Question

uble N-ethymaleimide-sensitive factor attachment THE SPACES PROVIDEDFOREACH QUESTION. membrane fusion machinery of intracellular conformations, open (active) and closed ( a process known as palmitoylation, where palm protein back to its inactive form. You discover a occur). No mutation in the amino acid sequence (nactive) (Figure 1). The transition between these conformations is controlled by r cell type with a depalmitoylation defect (depalmitoylation does not protei in receptor (SNARE) proteins are core constituents in the ransport. The protein YKT6 is a SNARE protein that exists in 2 different e by palmitoylation leads to active protein and depalmitoylation reverts the of YKT6 was observed nactive form Active form 1) Provide a hypothesis on YKT6 funct in tumor vs normal cells. (1 points) 2) How would you experimentally proveldisprove this hypothesis? (3 poin Palmitoylation Depalmitoylation Figure1: Adapted from Frédéric Daste et al. J Cell Sci 2015:128:4263-4272

Answer 1

1.ykt6 Is a snare protein involved in release of exosomes.Exosomes take part in cancer cell regulation.cancer cells overexpress exosomes.Overexpression of exosomes can increases metastasis and growth of cancer cells.it has been found that YKT6 regulates exosomes.

2. Experimentally if we target microaRNAs against YKT6 then it is possible to decrease the expression of YKT6 thereby decreasing the expression of exosomes.MicroRNA are noncoding RNA they target mRNA and negatively regulate its translation.they have ability to negatively regulate YKT6.this has shown to decrease the levels of exosomes in cancer cells.