How many actual crossovers are represented by each recombinant Sordaria ascus? What would happen if the...
How many actual crossovers are represented by each recombinant Sordaria ascus? What would happen if the other two chromatids also crossed over? Would you be able to tell if this happened when examining your squashes?
Homework Answers
A common lab experiment is to cross mutant Sordaria with the wild-type and observe the colouring ratios in the offspring. This experiment illustrates the concepts of genetic inheritance in a haploid organism. The ascus.contains 8 ascospores. Sordaria can give us information about crossing over during meiosis. If no crossing over then there is a 4:4 pattern. 4 black spores, and 4 tan spores all lined up. If crossing over does occur there is a 2:2:2:2 pattern visible, or a 2:4:2 pattern.
Therefore each recombinant ascus represents one crossover. If the other two chromatids also crossed over it would show the not change the 4:4 pattern as seen for no crossing over condition. We would not be able to distinguish these from non cross over phenotypes.
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