You would like to purify protein A (pI=6.0, MW=32 kDa) from a mixture that also contains...
You would like to purify protein A (pI=6.0, MW=32 kDa) from a
mixture that also contains protein B (pI=6.0, MW 32 kDa) and
peptide C (pI=5.5, MW= 3 kDa). Which one of the following
techniques would give you the best possible result?
a) High Pressure Liquid Chromatography (HPLC)
b) Gel Filtration
c)Ion Exchange
d)Affinity Chromatography
e) SDS-PAGE
Homework Answers
Ion exchange Chromatography -- answer
HPLC purification frequently results in denaturation of the purified proteins and is thus not applicable to proteins
Gel Filtration--- separated by their size/molaecular wt since two them have same this cant be used
SDS-PAGE --- protien is made into smaller parts
Affinity Chromatography--- highly specific interaction such as that between antigen and antibody, enzyme andsubstrate, or receptor and ligand
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