Question

Hi

Could anyone please post a lab report for the following topic " Southern Blotting'

The lab report should also have the following criteria Introduction, Aims, Principle, procedure, and results(if possible).

Answer 1

yIntroduction of southern blotting :- (With Aim) It is used in molecular biology lose the identification for: of protein and nucleic acid and mainly used gose diagnostic purpose. It is the main tools for the identification of biomolecule such as DNA, MANO protein during different stage of gene expression. + Purinciple of Southern blotting: to breage DNA into small Its main principle is wred fragment by vestiction endonuclease enzyme. There fragmenta are then separated using electrophoresis Ly Procedure of Southern blotting : gta main procedure is - YONA purification DNA is purified and seburated by alcohol precipitation and all fibres are moremoved by buffer lix Fragmentation - The long nucleotide chain must - be broken into Camaller part for puri -fication or identification Process ilix Gel electrophoresist Nucleic acid are negativel charged so they move towards the anode in an elechophoresis chamber ivy Denaturation - In it double shanded DHA is denatured in alkaline medium. and result in formation of denatured DNA wy Blotting gn it DNA molecule is saturated - using a Nal solution and permanently fixed wil Hybridization - In it labeled probe is added to membrane buffer and incubated for as it takes several hour Result - The he bridization of probe at specific DNA result to conclusion that probe is specific to fragment on membrane * Please like it -