1) Explain the process of loading protein ladder and actin/myosin protein onto the gel with the...
1) Explain the process of loading protein ladder and actin/myosin protein onto the gel with the fish samples and how it is necessary for analysis of the gel and immunoblot?
2) Why was the Bradford assay performed with the fish protein extracts? Fully explain why this was necessary for analysis of the gel and immunoblot.
3) The molecular mass of Myosin Light chain is approximately 22KD, myosin heavy chain is 210KD and actin is 42KD. Which protein will migrate the fastest through the gel and why?
Homework Answers
Answer of Question No 3 - The smaller protein i.e, myosin light chain approximately 22KD will migrate the fastest through the gel because smaller protein can move through the gel matrix more quickly .
Add Answer to:
1) Explain the process of loading protein ladder and
actin/myosin protein onto the gel with the...
1) Why was the membrane incubated in blocking buffer? What would be the consequence of failing...
1) Why was the membrane incubated in blocking buffer? What would be the consequence of failing to block the membrane? 2) Explain the process of loading protein ladder and actin/myosin protein onto the gel with the fish samples and how it is necessary for analysis of the gel and immunoblot? 3) Why was a Bradford assay performed with the fish protein extracts? Fully explain why this was necessary for analysis of the gel and immunoblot? 4) The molecular mass of...
Questions: 1)why was the membrane incubated in blocking buffer? What would be the consequences of failing...
Questions: 1)why was the membrane incubated in blocking buffer? What would be the consequences of failing to block the membrane? 2) The molecular mass of Myosin Light Chain is approximately 22 KD, myosin heavy chain is 210 KD and actin is 42KD. Which protein will migrate the fastest through the gel and why?
In the Western blot experiment, )Why are proteins treated with ionic detergent (SDS), reducing agents (DTT), and heat before SDS-PAGE Why do SDS-coated proteins migrate in an electric field? e...
In the Western blot experiment, )Why are proteins treated with ionic detergent (SDS), reducing agents (DTT), and heat before SDS-PAGE Why do SDS-coated proteins migrate in an electric field? e molecular mass of myosin light chain I is approximately 22 kD, myosin heavy chain is 200 kD ane actin is 42 kD, Which proteins will migrate fastest through the gel? Why?
In the Western blot experiment, )Why are proteins treated with ionic detergent (SDS), reducing agents (DTT), and heat before...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
In the Western blot experiment, )Why are proteins treated with ionic detergent (SDS), reducing agents (DTT), and heat before SDS-PAGE Why do SDS-coated proteins migrate in an electric field? e molecular mass of myosin light chain I is approximately 22 kD, myosin heavy chain is 200 kD ane actin is 42 kD, Which proteins will migrate fastest through the gel? Why?
In the Western blot experiment, )Why are proteins treated with ionic detergent (SDS), reducing agents (DTT), and heat before...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 10 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 10 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 10 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 10 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 10 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 10 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 10 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 10 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 10 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 10 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 10 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 10 months ago