Why would you use a column to remove the excess iron from an orange transferrin solution?
Why would you use a column to remove the excess iron from an orange transferrin solution?
Homework Answers
Solution:-
Sephadex G-25 gel filtration column is used to remove the excess iron from an orange transferrin solution
The behavior of FeCl3 and orange transferrin on a Sephadex G-25 gel filtration column shows the art that can be encountercd in the use of Fe3f salts. FeC13 at pH 3.5 and containing tracer amounts of radioactive 59Fe was applied to a Sephadex G25 column which was equilibrated and eluted with 5 mM Tris HCI buffer at pH 7.45. Analysis of the eluate indicated that the iron became firmly bound to the column matrix and was not eluted as a high or low molecular weight fraction.
FeC13 is able to bind to large molecules in a nonspecific fashion, and be transported through the column matrix.
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Why would you use a column to remove the excess iron from an
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