Question

This is a Biochemistry 200 level question, please answer neatly and as soon as possible. Thanks in advance, and I will like as soon as answer is posted!

The enzyme happyase catalyzes the following reaction: SAD — HAPPY An enzyme kinetics experiment gave the data shown in the table below. The concentration of happyase used in the experiment was 25.00 uM. [SAD] (MM) 1.000 2.000 3.000 4.000 Vo (mM/s) 3.700 6.727 9.250 11.385

Answer 1

for an enzyme catalysis reaction Rate = K [o] Eso] - u KM+Cso]. where, Kg = constanı [E ] = Initial cone of enzyme [s ] = substrate cone. Happyese SAD Cone. from the slope of tot vs Ro we will get KM. and from inter ce pr Vmax te k₂CEJ now, Yvol 127= 0.27 / +27 2014 Bg slope = cu y = 01207 -0.2414 Intercept . we know y=mxtc By putting the ay 0.27= 0.2414 x Lte xoy valme room the first = c = 0.27 - 0.2914 data set. -0.0286

Ka+C50] BEJ Cse] TRTO - Bikan tesc) » tag = tegasus ! WALE - cu tu ke + Cel. in the form y=mx+c (straight une) c = tid = Intereept Slope - KIE]. slope: Intercept (Eo] = final Enzyme conc. cso] - Initial substrate cone. to us teso] is known as line Plot is known as line weaver Barok plot.

2 024142 slope - = km = 0.0286 Intercopr = 8.44 KIE]. - Intercept = 0.0286 - 34.965 & go, from ea ů Rate = 34.965 tefte um X 16.8×10 un 8.44 + 1688103 un 587412 16888.44 unls .. = 34.41 unis = 0.0344 tn/mm/s in the desired rate of the reaction = 0.034 0 mms!