Question

11. (A). Why a buffer is commonly used in biochemical reactions? In protein isolation, protease inhibitors are commonly presence in buffer solution, why? (b). Draw the structure of ammonium sulfate. Ammonium sulfate in a protein solution is able to precipitate a fraction of proteins out of solution. Why? (c). How to define the "% saturation of ammonium sulfate"? Be specific 12. (a). Why the wavelength that gives maximal absorption is selected for colorimetric assay? (b). Beer's law is useful for determining the conc. of solute only if the relationship between absorbance and conc. is in a linear relationship. When the conc. of the solute is high this linear relationship doesn't exist, why?
please help with #12!!! please

Answer 1