HomeworkLib
Question

Species A and B were separated using column chromatography. The elution time for species A (tA)...

Species A and B were separated using column chromatography. The elution time for species A (tA) was 200 s with a peak width (wA) of 35 s, and the elution time for species B (tB) was 353 s with a peak width (wB) of 51 s. What is the length of the column (in centimeters) if the theoretical plate height for species B is 3.0 mm?

L=

science   chemistry   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

page-1 solution:- * Given data- > tb = 353.5 => WB = 51.5 =) Height of species B = 3.0mm To determine the length No. of platepage-2 =) L=3 mm x 766.53 ) L=2299.59 mm » L= 2299.59mmx 0.1cm Imm - L=229.96cm ~ 230cm. [L= 230cm) Hence, length is 230 cm (

0 0
Add a comment
Know the answer?
Add Answer to:
Species A and B were separated using column chromatography. The elution time for species A (tA)...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • 20 > Species A and B were separated using column chromatography. The elution time for species...

    20 > Species A and B were separated using column chromatography. The elution time for species A (A) was 200 s with a peak width (WA) of 35 s, and the elution time for species B (') was 382 s with a peak width (WR) of 42 s. What is the length of the column (in centimeters) if the theoretical plate height for species B is 1.0 mm? L =

  • EXERCISE 6: Two chemical species, A and B are separated by isothermal gas chromatography, using a...

    EXERCISE 6: Two chemical species, A and B are separated by isothermal gas chromatography, using a 2.00 m column having 5000 theoretical plates at a flow rate of 15.0 ml / min. The peak of unabsorbed air appears after 30 s; the peak of A appears after 5 min and that of B after 12 min (a) Calculate the dead volume VM of the column, and the retention volumes Va and VB. (b) Calculate the reduced volumes V’a and V'B...

  • Two compounds C and D are separated on a column 34 cm long . Given the...

    Two compounds C and D are separated on a column 34 cm long . Given the chromatographic data below calculate The column resolution? , the number of theoretical plates ?the height of theoretical plate and what length of column would be required to give a resolution of 1.5? Compound C retention time 18.3 width at base of peak(min) = 2.18 Compound D retention time 17.7, width at base 1.23

  • 3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min...

    3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min with a full width at half max (FWHM) of 1.20 s? The length of the column is 30.0 m. What is the plate height in microns? What is the peak resolution for this peak and another peak eluted at 4.73 minutes that has a full peak width (measured at the base) of 2.14 seconds? Is this sufficient for quantitative analysis of those two components?...

  • The table contains data for a liquid chromatography separation of four compounds, A, B, C, and...

    The table contains data for a liquid chromatography separation of four compounds, A, B, C, and D, on a 40.0 cm column Retention Compound time (min) Width at the base (min) A 5.17 0.436 B 1.02 13.0 15.0 C 1.10 23.4 1.91 Calculate the number of theoretical plates, N, for each peak. NA NB Nc ND = What is the average number of theoretical plates, Navg, for Navg the column? What is the plate height, H, for the column? Н-...

  • A mixture of two compounds (A and B) was separated by gas chromatography on a non-polar...

    A mixture of two compounds (A and B) was separated by gas chromatography on a non-polar column. The chromatogram was recorded on a chart recorder that fed paper at a constant speed of 100 mm per minute, and a mark was made on the chart when the sample was injected. The first peak on the chromatogram corresponds to compound A, and that peak was observed at a distance of 5.42 cm from the sample injection point, and it had a...

  • A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine,...

    A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, tc,was found to be 207.6 s with a baseline peak width, wc, of 16.2 s. The retention time for aspartame, ta, was 253.7 s with a baseline peak width, wa, of 18.7 s. The retention time for the unretained solvent methanol was 40.7 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.3 cmlong...

  • A peak elutes from a column at 31.8 min,31.8 min, with a width at half height...

    A peak elutes from a column at 31.8 min,31.8 min, with a width at half height of 10.1 s10.1 s. How many theoretical plates does this column have? N = ____________ plates The column length is 28.0 cm.28.0 cm. What is the plate height in millimeters? H = ___________ mm

  • Consider the data below for a liquid chromatography separation of four compounds, A, B, C, and...

    Consider the data below for a liquid chromatography separation of four compounds, A, B, C, and D on a 45.0 cm column. Calculate the number of theoretical plates, N, for each peak. Retention Width at the Time (min) Base (min) 5.24 0.424 Number Number 12.8 1.08 NA = II 14.2 1.12 D 24.1 1.85 Number Number NB = 10 N= What is the average number of theoretical plates, Navg, for the column? Number Navy = avg What is the plate...

  • The following data applies to a column for liquid chromatography: Use this information to answer the...

    The following data applies to a column for liquid chromatography: Use this information to answer the question below Length of column = 30.50 cm Flow rate = 0.313 mL/min A chromatogram of a mixture of species A, B, C, and D provided the following data: Species Retention time, minutes Width of peak base (W), minutes А 5.4 0.41 B 13.3 1.07 C 14.1 1.16 D 21.6 1.72 Calculate the resolution for species C and D Select one: O A 2.60...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT