Question

Experiment 6: Factors affecting enzyme catalyzed reactions Data Page Name: 1. STANDARD CURVE Table 1: Standard Curve Use your standard curve to calculate molar absorptivity Tube# Abs. mg/ml PNP coefficient of PNP? Show work. 1 0 2 0 :183 0.063 3 0.264 0.125 4 0.530 0.25 5 0.95 0.5 Pape 57

Answer 1

Table	Absorption	mg/ml PNP
1	0	0
2	0.133	0.063
3	0.264	0.125
4	0.530	0.25
5	0.95	0.5
6	2.10	1

We can write C insted of PNP.

PO = 1
In a graph of A vs. C, the slope is $\varepsilon d$

Slope = ed = 42—41_2.10 - 0 22 - 1-0 -= 2.10(mg/ml)

d = 1cm

2.10(mg/ml) - E = 2.10(mg/ml) 1ст

$= 2.10(mg/ml) cm

(92 12) 2 tot 0.95 0.5304 0. 2644 0.133 o. s i lu, y, 0.063 0.125 0.25 mg/me pop