What causes a “smiley face” gel? Why?
What causes a “smiley face” gel? Why?
Homework Answers
This problem occurs in gel electrophoresis.
When the voltage applied is too high according to gel concentration, it starts to heat up the center of the gel is hotter than the edges. It is also called as edge effect and it gives "smiley face"
It can be improved by decreasing the power setting and by checking the buffer.
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What separation method commonly uses a horizontal gel electrophoresis apparatus? Why?
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