Question

Real time PCR very accurately quantifies the amount of template DNA because it detects before any reagents become limiting Tor F Measurements of gene expression at the transcriptional level typically require making cDNA from mRNA Tor F. An important distinction between traditional Sanger (dideoxy) DNA sequencing and 2nd generation (NGS) DNA sequencing is that NGS gives longer DNA sequence "reads" Tor F.

Answer 1

1. Real time PCR accurately quantifies the amount of template DNA because it detects before any reagents become limiting – F

Explanation : In real-time PCR the amount of DNA is measured after each cycle via fluorescent dyes that yield increasing fluorescent signal in direct proportion to the number of PCR product molecules generated. Quantification of amount of template DNA in real time allows to monitor the expression of a particular gene.

2. Measurements of gene expression at the transcriptional level typically require making cDNA from mRNA – T

Explanation : The mRNA is a very unstable molecule. It is very diffuct to handle to perform any experiment. Moreover RNAses are ubiquitously present and there's a high chance that the RNA would get degraded. For using PCR, the methods are optimised to amplify DNA and not RNA. So, it is necessary to transform the mRNA into cDNA to perform any experiment regarding the measurement of gene expression.

3. An important distinction between traditional Sanger (dideoxy) DNA sequencing and 2nd generation (NGS) DNA sequencing is that NGS gives longer DNA sequence "reads" – T

Explanation : Next gen sequencing is a 2nd generation high throughput method of sequencing DNA using the concept of massively parallel processing. It can process millions of fragments simultaneously at a time and can efficiently sequence more or less 20 million basepair long fragments which is much more higher than that of the Sanger sequencing. So, the NGS can give longer DNA sequence "reads" comparing to the Sanger sequencing method.

**** IF YOU LIKE THE ANSWER PLEASE HIT THE THUMBS-UP. THANK YOU :-)