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You want to do RAC activation assay from your BJ cells and T cells. After that,...

  1. You want to do RAC activation assay from your BJ cells and T cells. After that, you want to compare the results? What technique should you use and how can you do that?

  2. Explain in your own words the technique

  3. Describe what is PAK1-PBD?

  4. show me the imaginary results using Control and Treated samples by drawing in PowerPoint or Paint? Consider that Treated cells have more Rac activation

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Answer #1

Ras-related C3 Botulinum Toxin Substrate 1 (RAC-1) belongs to the family of small Rho GTPases that are involved in variety of cell function. Serine threonine protein kinase-1 (PAK-1) has p21-binding domain (PBD) which binds to activated form of RAC, that is RAC-GTP. RAC activation can be assayed by affinity pull-down by PAK1-PBD and followed by western blot using anti-RAC antibody. Briefly, described in figure with the example of control and treated samples.

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