Question

The classic experiment that examined DNAse I sensitivity of chicken embryonic DNA from different tissues and at different developmental stages shows that: (Select all that apply.) A. the gene expression pattern changes during development. B. DNAse I sensitivity comes from sporadic mutations occurred during development. C. the chromatin structure changes in the course of development. D. DNAse I sensitivity only occurs in chicken but in no other organisms.

Key Experiment Question: Is chromatin structure altered during transcription? Method DNA's sensitivity to DNase I was tested on different tissues and at different times in development. Embryonic Adult H DNA highly globin gene globin genes sensitive Chicken to DNasel DNA Results Erythroblasts first 24 hours Erythroblasts 5 days clr Erythroblasts 14 days Brain cells throughout development Conclusion: Sensitivity of DNA to digestion by DNasel is correlated with gene expression, suggesting that chromatin structure changes during transcription.

Answer 1

Answer is option C.

The chromatin structure changes during transcription because sensitivity of DNA to digestion by DNase 1 is correlated with gene expression.