Question

Construct a graph representing the reversible unfolding, by urea, of a simple monomeric protein. Label the graph and describe the nature of the protein during unfolding as represented by the graph.

Answer 1

With increasing concentration of Urea the denaturant, initially the monomeric protein resist unfolding or denaturation.

SIGNAL CFluroscence intensity at 320 mm) 4 UREA CM) 8

But on gradual progression of molarity of Urea it starts unfolding and concentration of folded protein steeply decreases at around, urea molarity of 4 and almost all protein get unfold at molarity 8.