Question

. The mutations wad to non-functional anymes Y Ares active The following gene symbols for acoperen mutations have the usual meanings whether active enzymes z, Y. and A will be produced both in bance of an Genotype of for the present predict Without IPTG Without IPTG without IPTG with PTG with PTG with PTO

Answer 1

In haploid genotype i+, oc, z+, y+, a+  : operator (Oc) is constitutive it means lac operon express constitutively even when repressor is present and whether IPTG is absent or present.

Z+ produce functional β-gaalactosidase enzyme without Isopropyl-β-D-thiogalactopyranoside (IPTG)

Z+ (-IPTG) = +

Y+= produce functional permease enzyme without Isopropyl-β-D-thiogalactopyranoside (IPTG)

Y+ (-IPTG)= +

A+= produce functional β-galactoside transacetylase enzyme without Isopropyl-β-D-thiogalactopyranoside (IPTG)

A+ (-IPTG)= +

Z+ produce functional β-gaalactosidase enzyme with Isopropyl-β-D-thiogalactopyranoside (IPTG)

Z+ (+IPTG)= +

Y+= produce functional permease enzyme with Isopropyl-β-D-thiogalactopyranoside (IPTG)

Y+ (+IPTG)= +

A+= produce functional β-galactoside transacetylase enzyme with Isopropyl-β-D-thiogalactopyranoside (IPTG)

A+ (+IPTG)= +