Question

Effective Cancer Treatment (DNA Content vs Active) Active (UM) DNA content (% of control) 100 80 100 Effective Cancer Treatment (DNA Fragmentation vs. Active) IV. DNA Fragmentation (% control) 50 75 Active (UM)

ILLUSTRATE HOW A GEL WOULD LOOK BASED ON THESE RESULTS.

Answer 1

As per the information in table, with increase in treatment concentration of Active molecule, the total DNA content is decreasing. At 100uM concentration there is 80% reduction in DNA content compared to control (0uM).

The below graph shows increase in DNA fragmentation with increase in treatment concentration of active molecule.

Below is the illustration of gel based on the above scenario

At 0 uM - one single thick band (since it is un fragmented DNA)

At 1 uM - two bands which would be slightly lighter with respect to 0uM (since 20% reduction in DNA content due to active compound treatment). These bands would be ahead of 0 uM bands due to smaller in size with respect to 0uM bands

At 10 uM - four bands which would be slightly lighter with respect to 1uM (since 50% reduction in DNA content due to active compound treatment). These bands would be ahead of 1 uM bands due to smaller in size with respect to 1uM bands

At 100 uM - Five bands which would be slightly lighter with respect to 10uM (since 80% reduction in DNA content due to active compound treatment). These bands would be ahead of 10 uM bands due to smaller in size with respect to 10uM bands