Question

Genetics: include pictures please.(:

For each of the following steps of recombination at the molecular level, briefly describe what is happening and draw out what the chromatids look like (just the DNA, don't need to draw out the proteins), and what proteins are involved in each step. 1. Double strand break formation 2. Resection 3. First strand invasion 4. Formation of the double Holliday junction 5. Branch migration 6. Resolution

Answer 1

streps involve :

• nick formation
• strand displacement
• branch migration
• ligation
• rotation or resolution
• finally crosssover

Homologous secombination w เ ฟ ผT có bị đa endanuclease action nich in thes the strands which are on inner side) (2) SIA بافبفعله | displacement only on the inner sich.

3) SA to o Gw ligation of the strands and gap sealed in man This is called junction holiday

proteins involved in homologous recombination in E.coli

• recBCD are involve in recombintaion
• Rec B : 3'-5' helicase and nuclease
• Rec C : recognise chi site ( after recognisation the Rec cut the DNA at chi site )
• Rec D : 5'-3' helicase