Question

Body Ted 1 ust. 1 No Spattable Par Replace ont Paragraph Styles 2 4. A certain strain of E. coli has been noticed to acquire tolerance to salinity with time, scientists have doubted a gene that is being carried by a plasmid and transferred to this strain of E. coli by conjugation with another salt tolerant Gram-negative bacteria. To confirm their hypothesis, scientists have incubated both bacterial types in convenient conditions for conjugation. Suggest a method that allows you to test for the appearance of new transconjugant E. coli strains. Explain.

Answer 1

The scientists can test this by the following experiment-

Grow both the strains in lb containing high salt concentration and monitor the growth curve. The control of this experiment is growing the salt toleratant bacteria in a separate tube.

If the transconjugation has happened, the bacteria will grow it the saline media and if not, it won't grow.